Single-molecule sequencing of the desiccation-tolerant grass Oropetium thomaeum

Author(s): Robert VanBuren, Doug Bryant, Patrick P. Edger, Haibao Tang, Diane Burgess, Dinakar Challabathula, Kristi Spittle, Richard Hall, Jenny Gu, Eric Lyons, Michael Freeling, Dorothea Bartels, Boudewijn Ten Hallers, Alex Hastie, Todd P. Michael and Todd C. Mockler
Date of Publication: November 2015
Abstract:

Plant genomes, and eukaryotic genomes in general, are typically repetitive, polyploid and heterozygous, which complicates genome assembly. The short read lengths of early Sanger and current next-generation sequencing platforms hinder assembly through complex repeat regions, and many draft and reference genomes are fragmented, lacking skewed GC and repetitive intergenic sequences, which are gaining importance due to projects like the Encyclopedia of DNA Elements (ENCODE. Here we report the whole-genome sequencing and assembly of the desiccation-tolerant grass Oropetium thomaeum.

Citation: VanBuren, R., Bryant, D., Edger, P.P., Tang, H., Burgess, D., Challabathula, D., Spittle, K., Hall, R., Gu, J., Lyons, E., Freeling, M., Bartels, D., Ten Hallers, B., Hastie, A., Michael, T.P., & Mockler, T.C. (2015). Single-molecule sequencing of the desiccation-tolerant grass Oropetium thomaeum. Nature, 527(7579), 508-511.
Team(s): Plant Team